منابع مشابه
Xerografting as a Novel Procedure in the Management of Syngnathia
Congenital fusion of the maxilla and the mandible is a rare condition. The extent of the problem ranges from simple mucosal adhesion (synechia) to severe bony fusion (syngnathia). Here we present a patient with zygomatico-mandibular fusion who was treated at neonatal and infancy periods at three different stages without tracheostomy. Having had recurrence for two times, porcine grafting was per...
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250 BioTechniques Vol. 34, No. 2 (2003) be expected from an amplificationbased strategy. To our knowledge, this is the first time that such analysis has been conducted to assess the representation of a genomic library. In summary, we have developed a quick, facile protocol for constructing libraries from relatively small amounts of DNA. Using our method, we have shown that a representative, ran...
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The subtractive-hybridization procedure (see e.g. (1)) is a powerful procedure for obtaining cDNA populations which are enriched for upand down-regulated gene products. The method is based on the elimination of the common fragments within the cDNA populations being compared and is straight forward (1). Unfortunately, the analysis of the outcome is based on a screening hybridization assay and, h...
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During the cloning of a transcript of one member of a closely related gene family, the human 7-crystallin gene family (1), we encountered a novel artifact of the polymerase chain reaction: the formation of chimeric cDNA molecules. Our experimental strategy in cloning the human 7E-crystallin transcript was a common one (2): first strand cDNA synthesis on human lens RNA using AMV reverse transcri...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1989
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)84913-x